The effect of allergenicity of Artemisia aucheri flowering taps in guinea pigs

Allergic rhinitis or hay fever, is the most frequently occuring airway disease of our time. Therefore, the aim of this study was to evaluate of the allergenicity effect of Artemisia aucheri extract. In this research, flowrs of Artemisia aucheri were collected from Isfahan around, Iran in September 2012. Flowes were extracted using phosphate-buffered saline, PH 7.4. Three concentration of extracts (5%, 10%, 15%) were prepared. The experiments were intraperitoneally injection of the extracts 3 times, once every 10 days. The flowering taps allergenicity were detected using subcutaneous, skin test, serological and clinical tests. Tests were done on Hartley male guinea pigs. In treated and control groups the appearance of wheal, their diameter, serum IgE, eosinophilia, neutrophilia were compared. Means of triplicate measurements and standard errors were determined for each sample. Dates were analyzed using ANOVA test in the p <0.05. During the skin prick test, the maximum allergenic sensitivity was observed for %15 extracts, with an average wheal diameter of about 2.5 cm. The numbers of eosinophils and neutrophils were increased in the treated animals with extracts comparing control groups. The serum of treated animals with phosphate-buffered saline extracts contained more IgE than serum of control animals. Our results confirm that Artemisia aucheri is another allergenic plant. The confirmation of these aspects would facilitate the preparation of an effective extract, improving the diagnosis of the allergy to the Artemisia aucheri.